A single low dose of total body irradiation in combination with adoptive transfer of Mam-A2.4 CD8 T cells eliminates tumor and prevents relapse.

<p>(A) HLA-A2⁺ transgenic mice were vaccinated on days 0, 14, 28 and 42, with 100 µg Mam-A2.4 cDNA. Five days after the last vaccination, splenocytes were harvested and transferred i.p into tumor bearing SCID-beige mice immediately following the administration of 2.5 Gy TBI. (B) Tumor regression was monitored in SCID-beige mice that received either tumor only, 1×10⁷ Mam-A2.4 specific spleen cells alone, TBI (2.5 Gy) only or 1×10⁷ Mam-A2.4 specific spleen cells and TBI. Tumor regression was monitored using calipers every four days (n = 4 mice/group). (C) Survival curve of tumor-bearing mice. Day 0 indicates day of splenocyte transfer and TBI treatment. Data are representative of 3 independent experiments. <i>**P<0.01</i>.