T-bet and Eomes regulate tissue distribution and survival of the adoptive transferred T cells in tumor-bearing recipient mice.

<p>B6-LY5.2/Cr mice were challenged with 3×10⁵ B16F0 cells <i>i.d.</i> 6 days later, mice were irradiated at 500 rad. On day 7, the mice were adoptively transferred with 5×10⁵ WT, T-bet−/−, Eomes−/−, or T-bet/Eomes DKO pmel-1 T cells which have been cultured in Th1 condition for 3 days. (A) Splenocytes (SPL), lymph node (LN) and tumor infiltrating lymphocytes (TILs) were harvested from tumor-bearing mice 20 days after T cell infusion. The percentage of donor cells (CD45.2⁺) cells was determined by flow cytometry and the representative dot plots were shown. (B) The average percentage of donor cells (CD45.2⁺) in spleen was shown (N = 4 for each genotype). (C) The average percentage of donor cells (CD45.2⁺) in lymph nodes was shown (N = 4 for each genotype). (D) The average percentage of donor cells (CD45.2⁺) in tumor was shown (N = 4 for each genotype). Data are presented as mean ± SEM of four mice for each genotype. *P<0.05, **P<0.01 by Student’s t-test.