pT120 β-catenin is significantly decreased in TGN of prostate cancer samples.

<p>Immunohistological staining was performed with H102 (panel A) and pT120 (panel B) antibodies on serial section of tissues. 3 and 4 are higher magnification of 1 and 2, respectively. 1 and 3 are normal prostate tissue. 5 and 6 show that pT120 β-catenin predominantly distributes to plasma membrane and nucleus in tumor samples. Bars in panels 1 and 2 equal to 50 micrometer. (C) Scatter plot for pT120 staining pattern in normal and prostate cancer samples. Normal and tumor samples were counted for the “particle” staining pattern as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0033830#pone-0033830-g004" target="_blank">Fig. 4B</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0033830#pone-0033830-g005" target="_blank">5B3</a>. The number on Y-axis represents the average of two independent counts. A cutoff at 30 (dashline) is used to as arbitrary definition of positive and negative staining.