Validation of microarray data by qRT-PCR.

<p>Relative accumulation of (A) <i>PDF1.1</i> (At1g75830) and (B) <i>PDF1.2a</i> (At5g44420) mRNA in ten-day old seedlings of the three <i>35S:ERF5</i> and three <i>35S:ERF6</i> lines was measured by qRT-PCR. Relative Quantitation (RQ) values were calculated after normalization to <i>PEX4</i> expression levels. Each value is the mean of three technical replicates and the data are representative of three independent experiments. The RQ values of <i>ERF5</i> and <i>ERF6</i> in the empty vector lines are too low to be detected in the graph.