DENV-induced ER rearrangement requires viral protein synthesis.

<p>Mock-infected 2fTGH cells were (A) treated with 10 µg/mL actinomycin D (Act D) or (B) treated with 50 µg/mL cycloheximide (CHX), fixed and stained intracellularly for viral proteins E (green) and NS3 (red); nuclear staining (blue) was performed with DAPI. DENV-infected 2fTGH cells were (C) mock-treated or treated with (D) Act D or (E) CHX at the time of infection. Cells were fixed and stained as in A and B. Figures (F) and (G) are increased magnification of figures C and D, respectively. Black arrows point to lamellar stack-like structures and white arrows point to vesicle-like pore openings on the enlarged ER. Scale bar = 2 µm. Total magnification 600×.