Lovastatin treatment of DENV-infected cells does not inhibit ER rearrangement.

<p>Mock-infected 2fTGH cells were treated with 10 µM lovastatin for 12 h and were stained intracellularly for (A) SREBP-2 (green) and HMGCR (red). (B) Cells were also stained for DENV proteins E (green) and NS3 (red). 2fTGH cells were infected with DENV-2 and mock-treated for 12 h. Cells were fixed and stained intracellularly for (C) SREBP-2 (magenta) and (D) HMGCR (magenta), followed by DENV E (green) and NS3 (red) staining. Nuclear staining (blue) was performed using DAPI. DENV-infected 2fTGH cells were treated with 10 µM lovastatin at the time of infection and incubated for 12 h. Cells were fixed and stained as described above for (E) SREBP2 (magenta) and (F) HMGCR (magenta) and DENV E (green) and NS3 (red). Nuclear staining (blue) was performed using DAPI, and images were acquired as previously described. Total magnification 630×.