Mutation level in Myc31 and Myc24 transgenic mice.

<p>Bar graphs show mutations per 100,000 basepairs sequenced for various non-immunoglobulin genes in germinal center (GC) B cells of <i>MYC</i> transgenic mice. Data from the Myc31 line is shown on the left of each graph and the Myc24 line on the right of each graph. Sequence data from the first 1 kb downstream of the MYC P0 promoter is labeled Myc31 or Myc24. Sequence data from the first intron (including the end of the first exon) was done only in Msh2^−/−Ung^−/− dko Myc31 mice and is labeled Int31. A star above the bar indicates that the mutation frequency is significantly (p<0.05) above the background frequency of 1.6 mutations per 100,000 basepairs sequenced reported by Liu et al. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0039601#pone.0039601-Liu2" target="_blank">[14]</a> using a Chi-square test with Yate’s correction. A “0” in place of a bar indicates that no mutations were found in all sequences analyzed. An “N” in place of a bar indicates that the sample was not sequenced. (A) Splenic GC B cells sorted from Myc31 or Myc24 positive, B1-8 heterozygous mice immunized 16 days prior with NP-CGG. (B) Mutation frequency in Peyer’s patch GC B cells isolated from either wild type mice (red bars) or <i>Msh2</i>^−/−<i>Ung</i>^−/− double knockout mice (yellow bars) aged at least four months with non-autoclaved food and tap water.