Expression of endogenous <i>c-Myc</i> and transgenic <i>MYC</i> in B cells.

<p>(A, B, and C) Bar graphs represent the average expression level of endogenous <i>c-Myc</i> (top row of each panel) or transgenic <i>MYC</i> (bottom row of each panel) relative to <i>Hprt</i> expression in the same sample for either the Myc31 mouse line (left column in each panel) or the Myc24 line (right column in each panel). Error bars denote the standard error of the mean (SEM). Cell populations analyzed were: (A) Progenitor cells (B220⁻, CD43⁻, IgM^-), Pro B cells (B220⁺, CD43⁺, IgM^-), Pre B cells (B220⁺, CD43⁻, IgM^-), and Immature B cells (B220⁺, CD43⁻, IgM⁺) sorted from bone marrow cells of 4–5 week old mice; (B) Naïve (B220⁺, NIP^lo, FAS^-) and GC B cells (B220⁺, NIP^hi, FAS⁺) sorted from spleens of mice immunized 16 days previously with NP-CGG; and (C) Naïve B cells (B220⁺, CD19⁺, PNA^lo), and GC B cells (B220⁺, CD19⁺, PNA^hi) sorted from Peyer’s patches isolated from mice that had lived at least 1 month with non-autoclaved food and tap water. Data represent an average of 2–6 experiments: Panel A top row, 4; panel A bottom row, 2; panel B top row, 6; panel B bottom row, 3; panel C top left, 6; panel C top right, 4; panel C bottom left, 5; panel C bottom right, 3.