Single nucleotide polymorphism (SNP) analysis of mutant mice.

<p>(A) Mutant mice on C57BL/6 background were crossed to C3H and the offspring (F1) was used for an intercross yielding F2 animals. Blood of these animals was analyzed for the percentage of T cells (TCR-β ⁺ DX5^-) and B cells (CD19⁺MHCII⁺). F2 animals were grouped according to the presence (unaffected) or absence (affected) of T and B cells and affected animals were used for SNP-analyses. (B) Graphical representation of SNP distribution in randomly selected F2 and control animals. SNPs derived from C57BL/6 are in blue, SNPs from C3H are in red and heterogenous SNPs are yellow. Shown are only informative SNPs at the beginning of chromosome 2. The chip contained 377 SNP of which 244 were informative in our cross. In total we performed SNP-analyses from 57 F2 animals as well as from 6 F1, C57BL/6 and C3H control animals each.