Interrelations between antibody mutations and immunological gene-expression in single B-cells.

<p>Normalized gene expression values (red denotes up-regulation and blue denotes down-regulation) were hierarchically-clustered across 193 single PE+ and PE- B-cells belonging to BALB/c (WT) and TCRd−/− (KO) mice, and plotted alongside mutational content of antibody heavy- and light-chains expressed by each (<b>A</b>). For the latter, cells for which light-chains either could not be sequenced or were rejected by the quality-filter are color-coded on the bar-plot in grey. Spearman-correlations were calculated between each gene-expression value and mutation-count for wild-type (<b>B</b>) and knock-out (Figure S3A in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0067624#pone.0067624.s001" target="_blank">File S1</a>) mice. Data were further analyzed by calculating differences between Spearman correlations performed on non-synonymous and synonymous-mutations separately (<b>C</b>), with absolute values of these differences depicted on the vertical axis.