Splenic cDC subset development and TLR2 expression is independent of IRF7.

<p>The presence of splenic CD11c^hiMHCII^hi cDCs in C57BL/6 and B6.<i>Irf7</i>^−/− mice was determined by flow cytometry (<b>A</b> & <b>B</b>). CD4⁺, DN and CD8α⁺ cDC subset frequency was determined in the CD11c^hiMHCII^hi compartment of wildtype and IRF7-deficient animals (<b>C</b> & <b>D</b>). Expression of TLR2 on CD11c^hiMHCII^hi cells from C57BL/6 and B6.<i>Irf7</i>^−/− mice was determined by flow cytometry (<b>E</b> & <b>F</b>). Flow plots and histograms in <b>A</b>, <b>C</b> and <b>E</b> are representative, data in <b>B</b>, <b>D</b> and <b>F</b> show mean frequency ± SEM of indicated cell population in C57BL/6 (open bars) and B6.<i>Irf7</i>^−/− (closed bars) mice. Histograms in <b>E</b> are gated on CD11c^hiMHCII^hi cells gated as in <b>A</b> and show specific TLR2 staining (open line) compared to isotype control antibody (filled histogram). Data are from n = 4–5 mice per group and representative of three separate experiments.