The plasmacytoid DC subset is expanded in young TC mice.

<p>Splenic DCs were isolated from 2-3 month old TC and B6 mice. <b>A</b>. Representative FACS plots showing the percentage of (<b>B</b>) myeloid DCs (CD11c⁺ CD8⁻) and (<b>C</b>) lymphoid DCs (CD11c⁺ CD8⁺). <b>D</b>. Representative histogram of DCIR2⁺ expression on CD11c⁺ splenic DCs in B6 (black line) and TC (dotted line) mice. The shaded histogram shows the isotype control and the horizontal line the gate for DCIR2⁺ DCs. The percentage of splenic CD11c⁺ DCs expressing DCIR2 in each strain is shown in (<b>E</b>)<b>. F</b>–<b>G</b>. Plasmacytoid DCs were identified as CD11c^mid PDCA-1⁺. Graphs show data from two experiments that were normalized to B6 means. Significance levels of two-tailed Mann-Whitney tests are shown.