<p>(<b>A</b>) Sorting strategy and representative sort purities of unfractionated cDCs (top left panel) and each sorted cDC subset. (<b>B</b>) IL-12p70 secretion by CD11c<sup>hi</sup>MHCII<sup>hi</sup> cDCs from naïve and/or day 28 infected mice, in the presence or absence of LPS. (<b>C and D</b>) Secretion of IL-12/23p40 by cDC subsets in the absence (B) or presence (C) of LPS. (<b>E and F</b>) Spontaneous IL-27p28 secretion by unfractionated cDCs (E) and sorted cDC subsets (F). (<b>G and H</b>) IL-10 secretion by unfractionated cDCs (G) and sorted (H) cDC subsets.</p
<p><i>A</i>, <i>B</i>, <i>C</i> and <i>D</i>. Following DC maturation, supernatant culture was colle...
<p>Flow cytometric analysis of pro-IL-1β- (<b>A</b>-<b>C</b>) or IL-6-expressing CD11c<sup>+</sup>MH...
<p>Representative flow cytometric analysis outlines the method used for delineation of subsets. This...
<p>The presence of splenic CD11c<sup>hi</sup>MHCII<sup>hi</sup> cDCs in C57BL/6 and B6.<i>Irf7</i><s...
<p>(<b>A</b>) Splenic cDCs were gated as MHCII<sup>hi</sup>CD11c<sup>hi</sup> cells (top panel) and ...
<p>CD11c<sup>hi</sup> cDCs were sorted from spleens of C57BL/6 and B6.<i>Irf7</i><sup>−/−</sup> mice...
<p>Splenocytes obtained from mice uninfected (UI) or infected 12 hours earlier with 10<sup>7</sup> c...
<p>DC subsets were isolated from spleens of BCG infected (i.v.) mice at day 21 post-immunization as ...
<p>B6 and <i>Il10</i><sup>−/−</sup> mice were infected with 5×10<sup>6</sup> PFU <i>Δm157</i> MCMV. ...
<p>Splenic DCs were isolated from 2-3 month old TC and B6 mice. <b>A</b>. Representative FACS plots ...
<p>BALB/c mice were treated with 1 µg/mouse IL-12 (solid square) or vehicle control (VC; open triang...
<p>Leukocytes from IL-12 or vehicle control treated mice were incubated with 100 µg/ml DQ-OVA for 30...
<p>Spleen (SPL) (<b>A</b>) and skin draining lymph nodes (SDLN) (<b>B</b>) of 8 weeks old WT and DKO...
<p>Splenocytes from naïve mice fed a high fat diet were treated with 20μg/ml tCRAMP for 4 or 24 hour...
<p>(A) Mice were infected with 2×10<sup>7 </sup><i>L. donovani</i> amastigotes i.v. and sacrificed a...
<p><i>A</i>, <i>B</i>, <i>C</i> and <i>D</i>. Following DC maturation, supernatant culture was colle...
<p>Flow cytometric analysis of pro-IL-1β- (<b>A</b>-<b>C</b>) or IL-6-expressing CD11c<sup>+</sup>MH...
<p>Representative flow cytometric analysis outlines the method used for delineation of subsets. This...
<p>The presence of splenic CD11c<sup>hi</sup>MHCII<sup>hi</sup> cDCs in C57BL/6 and B6.<i>Irf7</i><s...
<p>(<b>A</b>) Splenic cDCs were gated as MHCII<sup>hi</sup>CD11c<sup>hi</sup> cells (top panel) and ...
<p>CD11c<sup>hi</sup> cDCs were sorted from spleens of C57BL/6 and B6.<i>Irf7</i><sup>−/−</sup> mice...
<p>Splenocytes obtained from mice uninfected (UI) or infected 12 hours earlier with 10<sup>7</sup> c...
<p>DC subsets were isolated from spleens of BCG infected (i.v.) mice at day 21 post-immunization as ...
<p>B6 and <i>Il10</i><sup>−/−</sup> mice were infected with 5×10<sup>6</sup> PFU <i>Δm157</i> MCMV. ...
<p>Splenic DCs were isolated from 2-3 month old TC and B6 mice. <b>A</b>. Representative FACS plots ...
<p>BALB/c mice were treated with 1 µg/mouse IL-12 (solid square) or vehicle control (VC; open triang...
<p>Leukocytes from IL-12 or vehicle control treated mice were incubated with 100 µg/ml DQ-OVA for 30...
<p>Spleen (SPL) (<b>A</b>) and skin draining lymph nodes (SDLN) (<b>B</b>) of 8 weeks old WT and DKO...
<p>Splenocytes from naïve mice fed a high fat diet were treated with 20μg/ml tCRAMP for 4 or 24 hour...
<p>(A) Mice were infected with 2×10<sup>7 </sup><i>L. donovani</i> amastigotes i.v. and sacrificed a...
<p><i>A</i>, <i>B</i>, <i>C</i> and <i>D</i>. Following DC maturation, supernatant culture was colle...
<p>Flow cytometric analysis of pro-IL-1β- (<b>A</b>-<b>C</b>) or IL-6-expressing CD11c<sup>+</sup>MH...
<p>Representative flow cytometric analysis outlines the method used for delineation of subsets. This...