CTO inhibits the adhesion of LAMA84R cells to exosome-treated HUVEC monolayer.

<p>(a) Phase contrast micrographs showing the adhesion of LAMA84R cells (arrows) on HUVEC monolayer treated with 50 µg/ml of exosomes (Exo) and with 50 µg/ml of exosomes after pre-treatment of 24 h with 10 µM CTO. (b) Adhesion of LAMA84R cells to endothelial cell monolayer treated for 6 h with: 50 µg/ml of exosomes (Exo) and exosomes plus increasing doses of CTO (1–10 µM); (c) Adhesion of LAMA84R cells to endothelial cell monolayer treated for 6 h with: 50 µg/ml of exosomes (Exo), 50 µg/ml of exosomes plus 10 µg/ml of a neutralizing anti-IL8 antibody (N Ab IL8), 10 ng/ml of recombinant IL8 (Rec IL8), 10 ng/ml of recombinant IL8 plus 10 µg/ml of a neutralizing anti-IL8 antibody and 10 ng/ml of recombinant IL8 with increasing doses of CTO (1–10 µM). Values are representative for three independent experiments. *p≤0.05; **p≤0.01.