Monoclonal antibody inhibition of IL-8 and CXCR2 decreased TFF3 stimulated HUVEC tubule formation <i>in vitro</i>.

<p>(A) and (B), HUVEC tubule formation <i>in vitro</i> in the Matrigel after 12 hours co-culture with MCF-7 cells with forced expression of TFF3 treated with IgG control or 50 μg/mL of anti-IL-8 monoclonal antibody in serum-free conditions. IgG was used as control. HUVEC co-cultured with MCF7-Vec treated with IgG control was a baseline. Total tubule length (A) and tubule number (B) was assessed using ImageJ analysis software. (C) Representative light photomicrographs of HUVEC tubule formation <i>in vitro</i> in the Matrigel, in which HUVEC co-cultured with MCF7-Vec and MCF7-TFF3 treated with IgG control or 50 μg/mL of anti-IL-8 monoclonal antibody. **, <i>P</i> < <i>0</i>.<i>01</i> as compared to HUVEC co-cultured with MCF7-Vec or MCF7-TFF3 treated with IgG control. (D) and (E) HUVEC tubule formation <i>in vitro</i>, in which MCF-7 cells with forced expression of TFF3 co-cultured with HUVEC treated with IgG control or 20 μg/mL of anti-CXCR2 monoclonal antibody. MCF7-Vec co-cultured with HUVEC treated with IgG control was as baseline. Total tubule length (D) and tubule number (E) was assessed. (F) Representative light photomicrographs of HUVEC tubule formation <i>in vitro</i> in the Matrigel, in which MCF7-Vec and MCF7-TFF3 co-cultured with HUVEC treated with IgG control or 20 μg/mL of anti-CXCR2 monoclonal antibody. **, <i>P</i> < <i>0</i>.<i>01</i> as compared to MCF7-Vec or MCF7-TFF3 co-cultured with HUVEC treated with IgG control. Scale bar, 200 μm.