Silencing of Exo70 leads to Cav1 accumulation in focal adhesions.

<p>Mock-treated (<b>A</b>) or Exo70-depleted cells (<b>B</b>) were detached for 1 h and replated on fibronectin-coated substrate for 3 h, and then fixed and stained for endogenous vinculin and Cav1. Scale bars, 5 µm. Co-localization between Cav1 and vinculin was quantified in a 20-pixel width region from the cell periphery and compared in Exo70-depleted with two independent siRNAs <i>vs</i>. mock-treated cells (<b>C</b>). <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0052627#s2" target="_blank">Results</a> in panel C are the average of mean percentage of co-localization ± s.e.m. of three experiments. ** <i>P</i><0.05. Experiment has been repeated three times with number of cells analysed: n = 25 mock cells, n = 9 for cells treated with Exo70 siRNA 7c, and n = 23 for siRNA 7d.