Relative expression level of several genes involved in oxidative stress response and lipid metabolic pathways in Dnmt1^N/+ mice and Dnmt1^+/+ mice fed alcoholic or control diet.

<p>The fold changes were compared to the expression level in the wild type mice fed control diet, which was assigned an arbitrary value of 1.</p><p>qPCR analysis was performed using gene-specific primers, each sample was analyzed in triplicate and the data was normalized to 18S rRNA (n = 5). The primer sequences are provided in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0041949#pone-0041949-t001" target="_blank">Table 1</a>. Relative expression was calculated using the ΔΔC_T method (15). Statistical analysis of the qPCR data was performed using the student’s 2-tailed t test. * Represents significant changes in hepatic gene expression in mice fed alcoholic diet compared to those fed control diet, ≠ denotes significant changes in the hypomorphic mice compared to wild type mice both fed alcoholic diet and # indicates gene with significantly less expression in the mutant mice compared to the wild type mice both fed control diet.