Expression of oscillating metabolic genes in the liver of wild-type C57BL/6J mice.

<p>Gene expression of A) <i>Acaca</i>, B) <i>Adh1</i>, C) <i>Cpt1a</i>, D) <i>Cyp2e1</i>, E) <i>Dgat2</i>, F) <i>Nampt</i>, G) <i>Pck1</i>, H) <i>Pdk4</i>, I) <i>Ppargc1a</i>, J) <i>Ppargc1a</i>, and K) <i>Srebp1c</i> in livers from mice fed control (♦) or ethanol-containing (grey square) liquid diets was determined using real-time quantitative PCR. Expression levels were normalized to the <i>Gapdh</i> housekeeping gene and are displayed as fold-change from a single control mouse. Cosinor analysis for curve fitting was performed using the nonlinear regression module in SPSS. Cosinor analysis results are included in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0071684#pone-0071684-t003" target="_blank">Table 3</a>. Data are presented as mean ± SEM for n = 4–6 control and ethanol-fed mice at each time point.