<i>Dnmt1</i>^N/+ mice exhibit reduced hepatic Dnmtase activity compared to the wild type mice fed control diet, which is further reduced upon feeding alcohol diet for 6 weeks.

<p><b>A.</b> qRT-PCR analysis of Dnmt1 in the livers of the wild type (+/+) and mutant (N/+) mice. <b>B.</b> Western blot analysis of Dnmt protein levels in the liver extracts of wild type and hypomorphic mice fed control or alcohol diet. C. Quantitative analysis of the western blot data in B by ImageJ software. Gapdh normalized signal of Dnmt1 and Dnmt3b in the wild type livers was assigned a value of 1. <b>D.</b> Dnmtase activity in the hypomorphic liver nuclear extracts was significantly reduced in alcohol fed mice. Dnmtase activity was measured as described in Fig. 1A. Single, double and triple asterisks represent P-values ≤0.05≤0.01 and ≤0.005, respectively. <b>E</b> Global DNA methylation (GDM) in the liver DNA was not altered in wild type and mutant mice fed alcohol diet. Briefly, genomic DNA from the liver was enzymatically hydrolyzed to nucleosides followed by LC-MS/MS analysis. GDM is represented as a ratio of 5-mdC to total cytosine. 5mdC and 2dC denote 5-methyl-2-deoxycytidine and 2-deoxycytidine, respectively.