Mutating a putative AEP cleavage site abrogates TLR7 signaling.

<p>(A) Immunofluorescence microscopy of resting (top) or imiquimod activated (bottom) fibroblast co-transfected with FL or N478Q TLR7 HA-tagged and UNC93B1-cherry cDNAs and stained for TLR7 (green), LAMP1 (red) and UNC93B1 (red). One representative experiment out of three shown. (B) Quantification of colocalization using Image J software (n = 8). (C) KC secretion in fibroblasts transfected with FL or N478Q TLR7and stimulated overnight with 10 µg/mL imiquimod. (n = 2; mean ± SEM, * p<0.05). (D) IL-6 secretion in TLR7^−/− BMDCs transfected with FL or N478Q TLR7 and stimulated with TLR ligands as above. (n = 4; mean ± SEM, ** p<0.01).