TLR7 C-ter is functional and requires acidic environment.

<p>(A) IL-6 secretion in TLR7^−/− BMDCs transfected with pcDNA3.1, FL, C-ter or N-ter TLR7 cDNAs and stimulated with 5 µg/mL of imiquimod or 10 ng/mL of LPS for 16 h. (n = 3–4; mean ± SEM, * p<0.05, ** p<0.01). (B) IL-6 secretion in TLR7^−/− BMDCs transfected with FL or C-ter TLR7 and stimulated with imiquimod as above and treated with or without 25 µM MV026630 (AEP inhibitor). (n = 3; mean ± SEM, * p<0.05). (C) IL-6 secretion in BMDCs activated with either 5 µg/mL imiquimod or 10 ng/mL LPS for 16 h, in the presence or not of 20 nM of concanamycin B. (n = 3; mean ± SEM, ** p<0.01, *** p<0.001). (D) IL-6 secretion in TLR7^−/− BMDCs transfected with FL or C-ter TLR7 and stimulated with imiquimod and treated as above. (n = 3; mean ± SEM, * p<0.05).