Stabilizing E2f1 during S phase induces <i>hid</i> expression.

<p>A, B) qRT-PCR quantification of <i>hid</i> mRNA (A) or <i>rpr</i> mRNA (B) in en-Gal4 wing discs expressing GFP or the indicated GFP-E2f1 fusion proteins that lack (grey) or contain (black) the PIP3A mutation relative to a non-transgenic <i>w¹¹¹⁸</i> control (Con). * p<0.001. C–I) Detection of apoptosis via Cleaved Caspase-3 (CC3, red) staining of third instar larval wing imaginal discs expressing the indicated GFP-E2f1 (GFP, green) proteins with en-Gal4. En-Gal4>E2f1^Stable (C–E) or E2f1^Stable/DBD (F–H) in either a wildtype <i>hid</i> background (+/+), or heterozygous <i>Hid⁰⁵¹⁴¹</i>/+ or <i>Df(3L)H99</i>/+ backgrounds. I) Quantification of CC3 pixel intensity as measured using ImageJ. All genotypes were normalized against E2f1^Stable; +/+ cleaved caspase-3 levels. * p<0.001. n.s. not significant. n = 12 discs for each genotype.