Induction of Apoptosis requires full-length E2f1^Stable.

<p>A) Schematic of the E2f1^336–805 mutant protein, which contains an NH₂-terminal HA tag. B) Detection of apoptosis via Cleaved Caspase-3 (CC3, red) staining of third instar larval wing imaginal discs expressing HA-E2f1^336–805 (anti-HA, green) with en-Gal4. Bar = 50 µm. C) Anti-E2f1 western blot of third instar imaginal wing discs expressing GFP-E2f1, GFP-E2f1^Stable, or HA-E2f1^336–805. D) Co-immunoprecipitation analysis of Myc-E2f1 and HA-Rbf1 from transiently transfected S2 cells.