Cell death and protection in discs with clonal Hid/Rpr expression.

<p>The larvae were generated as described for <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004220#pgen-1004220-g001" target="_blank">Figure 1</a> and irradiated with 4000R of X-rays after de-repression of GAL4. Wing imaginal discs were extirpated 4 h after irradiation and fixed and stained for DNA (A, E) and cleaved active Caspase 3 (B, F). RFP clonal marker (C, G) was used to mark the clonal boundaries in the images. (D, H) show merged images. (I) shows the timeline followed. * shows areas outside the clone that display robust caspase activation in RFP only discs (top row) but not in RFP, Hid/Rpr discs (bottom row).