Overexpression of <i>da</i> mimics the phenotype induced by the absence of <i>emc</i>.

<p>(<b>A, B</b>) Third instar wing imaginal discs with Flip-out control (A) and <i>UAS-da</i> (B) clones labelled with GFP (green) and stained for Wingless (Wg, in red). Clones of <i>da</i>-expressing cells were smaller than the control clones (compare B to A). (<b>C, D</b>) Third instar <i>sal^EPv-Gal4 UAS-GFP/+</i> (C) and <i>sal^EPv-Gal4 UAS-GFP/UAS-da</i> (D) wing imaginal discs stained for the mitotic marker phospho-histone-3 (PH3). This marker was strongly diminished in the <i>sal^EPv</i> expression domain (in green) of <i>da</i>-expressing discs. Accordingly, this region was smaller in mutant versus control discs (compare D with C). (<b>E, F</b>) FACS analysis of third instar wing imaginal discs of the <i>sal^EPv-Gal4 UAS-GFP/+</i> (E) and <i>sal^EPv-Gal4 UAS-GFP/UAS-da</i> (F) genotypes. Note the accumulation of GFP ⁺ cells from the <i>UAS-da</i> expressing discs in the G2 phase (F, n = 3 independent experiments, p-value<0.05).