miR290-5p and miR292-5p are induced at the pro-B to pre-B transition.

<p>1A. Heat-map representing levels of miRNAs from a microarray analysis of RNA purified from the indicated AMuLV cell lines cultured in the absence or presence of STI571 (2.5 µM, 12 hr). Performed once in three independently transformed cell lines. 1B. Schematic depiction of the shared seed sequence of the mature miR290-5p and miR292-5p microRNAs. 1C. qPCR analysis of miR290-5p or miR292-5p expression levels in RNA purified from E2A+/+ AMuLV cells cultured in the absence or presence of STI571 (2.5 µM, 12 hr). Data was normalized to the expression of miR129-2_3p. Error bars represent range for replicate qPCR reactions. The data shown is from one representative experiment of three biological replicates. 1D. qPCR analysis of miR290-5p or miR292-5p in primary wild-type pro-B (B220+, CD43+, IgM−) cells or pre-B (B220+, CD43−, IgM−) cells. Data was normalized to the expression of miR129-2_3p. Error bars represent range for replicate qPCR reactions. Data shown is from one representative experiment, of three independent sort experiments.