κGT induction is blunted upon knockdown of miR290-5p or miR292-5p.

<p>3A. qPCR analysis of κGT expression in RNA purified from E2A+/+ AMuLV cells expressing a miR290-5p or miR292-5p sponge knockdown construct, and cultured in the presence of STI571 (2.5 µM) for the indicated lengths of time. Data was normalized to the expression of <i>Hprt</i>. Error bars represent range for replicate qPCR reactions. Asterisk represents P value <0.05. The P value was derived by the Student’s T test. These data are from one representative experiment of at least four independently performed experiments. 3B, C. qPCR analysis of (B) κGT or (C) Rag1 expression in RNA purified from wild-type primary pro-B cells transduced with either a miR129-2_3p control knockdown sponge, or a miR290-5p or miR292-5p knockdown sponge. Wild-type primary pro-B cells were cultured in rIL7 (2 ng/ml) for 5 days and then transduced with indicated sponge construct. Marker positive cells were then sorted and IL7 withdrawn from culture for 24 hrs before RNA was harvested. qPCR measures (B) κGT or (C) Rag1 expression in cells before or after the withdrawal of IL7. Data was normalized to <i>Hprt</i> expression levels. Error bars represent range for replicate qPCR reactions. Asterisk represents P value <0.05. The P value was derived by the Student’s T test. These data show one representative experiment of at least four independently performed experiments.