MiR-129-5p can be induced by IFN-β in Hela cells.
- Jiarong Zhang (223696)
- Shuangdi Li (497784)
- Qin Yan (281622)
- Xiaoyue Chen (334717)
- Yixia Yang (497785)
- Xuelian Liu (497786)
- Xiaoping Wan (428649)
Publication date
December 2013
DOIAbstract
<p>(A) Validation of the microarray data by qPCR. Triplicate assays were performed for each sample. Inductions of known IFN-regulated genes <i>ISG54</i> and <i>ISG56</i> are shown for comparsion. (B) Time course of miRNA induction by IFN-β. Hela cells were stimulated with 300 IU ml<sup>−1</sup> IFN-β for the indicated times, and <i>miR-129-5p</i> and <i>ISG54</i>/<i>ISG56</i> expressions were quantified by qPCR. (C) Dose-response analysis of miRNA induction by IFN-β. Hela cells were stimulated with the indicated doses of IFN-β for 2 h, and <i>miR-129-5p</i> and <i>ISG54</i>/<i>ISG56</i> expressions were quantified by qPCR.</p
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