miRNA expression profiling of LX2 cells.

<p>LX2 cells were treated with virus-free culture supernatants from mock, R5- or X4-tropic HIV-1 infected PBMCs for 72 hr. Total RNA was isolated and subjected to miRNA profiling as described in Methods. (A) Percent distribution of C_T values for the 1008 miRNAs across the samples. Any C_T value above 35 was taken as 35. Entire profiling was done in set of three and data represents mean ± S.E. of three independent experiments. (B) Heat map depicting relative expression levels of miRNAs that showed significant changes across all the biological replicates. The heat map shows hierarchical clustering of miRNAs. Each row represents miRNA and each column represents samples tested. Red and green represent miRNAs with expression levels above and below the mean, respectively.