Reduced recruitment of myeloid BMDCs in DmiR expressing tumors.

<p>RT-PCR analysis of Bv8 level in DmiR over-expressing cells (A). All three DmiRs potently inhibit the expression of Bv8. Evaluation of Gr1 and CD11b positive bone marrow-derived myeloid cells in DmiR vs. control (GFP) expressing A-GBM tumors (B). MiR-588-GFP or vector-control- GFP expressing A-GBM tumors were stained for Gr1/CD11b myeloid cell- or CD31 vascular endothelial marker. Images of the same plain with detectors for GFP or antibody staining are shown. Scale bar represent 20 µm. Tumors were collected 73 days following injection to mice. Images were taken as representatives of at least three different tumors per group. Impaired mobilization of Gr1 and CD11b positive myeloid cells in circulation of miR-588 tumor bearing mice (C). Percentage of Gr1 or CD11b expressing cells among CD45 positive cells in circulation of mice bearing fast-growing glioblastoma expressing either GFP (black bars) or miR-588 (dotted bars) was determined using FACS analysis. Error bars represent SEM, ** p<0.02.