Increased fraction of pre B-cells in BM cells of miRNA-expressing mice.

<p>The immuno-phenotypic profile of BM cells harvested from tibiae and femora was characterized by flow cytometry. A) Decrease of myeloid population in miRNA-expressing mice. The graph represents flow cytometry results of GFP⁺ gated BM cells (total number of mice: control gfp: n = 12; kshv-miR-K12-11: n = 16; hsa-miR-155: n = 13). The result show a decrease of myeloid cell populations (CD11b⁺Gr1⁺) in the miRNA-expressing mice compared to control mice. B) Increase of B-cell population in the BM cells of miRNA-expressing mice. GFP⁺ gated BM cells from miRNA-expressing mice reveal a slight increase of B-lineage cells compared to GFP control mice (B220⁺ (left graph; (control gfp: n = 13; kshv-miR-K12-11: n = 11; hsa-miR-155: n = 16)) and CD19⁺ (right graph; (control gfp: n = 10; kshv-miR-K12-11: n = 9; hsa-miR-155: n = 13)). C) CD43 expression evaluated by flow cytometry on GFP⁺/CD19⁺ gated BM cells reveals a significant increase of the pre B-cell population (CD19⁺CD43⁻) in the BM of miRNA-expressing mice, indicating a shift toward the pre B-cell fraction. Left: histograms of one representative mouse each of miRNA-expressing and control mice. Gate P1 indicates the CD43⁻ population. Right: dot plots of all analyzed mice (control gfp: n = 10; kshv-miR-K12-11: n = 9; hsa-miR-155: n = 13).