TUNEL staining combined with immunohistochemical labeling of astrocytes in adult organotypic brain slices.

<p>Apoptotic cell nuclei (green), astrocytes (red) and cell nuclei (blue) were visualized by TUNEL staining, anti-GFAP and Hoechst, respectively, in the somatosensory cortex (layer VI) of directly fixed (30 µm) and sub-sectioned (30 µm) cultivated brain slices of adult P301S mice (wt/tg mixed). A–C: directly fixed tissue; D–F: tissue treated with staurosporine and fixed on 1^st DIV; G–I: tissue fixed on 1^st DIV; J–L: tissue fixed on 15^th DIV. A, D, G, J: TUNEL; B, E, H, K: GFAP; C, F, I, L: merged images of TUNEL, GFAP and Hoechst. Scale bar 20 µm.