Detection of apoptotic cells in the dentate gyrus.

<p>Representative examples of Hoescht 33342-counterstained (A, C, E, G, I (blue)) TUNEL label (B, D, F, H, I (green)) in the dentate gyrus (A–F) or cerebral cortex (G–I) of P14 control (A, B, E–I) and MD+NIC (C, D) treated animals. Sections were treated with DNase 1 to generate DNA strand breaks, as a positive control (B). There was no TUNEL positive reaction in either NIC+MD (D) or in CTL (F) P15 DG. More superficial cortical layers had numerous cells that were positive for TUNEL as part of normal development (H, I). G–I served as a same section control for DG regions shown in E, F. Arrows point to endogenous TUNEL+ cells that have little to no reactivity for the Hoescht nuclear stain. Bars = 50 µm for all. I, II, III represent layers I, II, III of the cerebral cortex.