Immunohistochemical labeling of neurons and astrocytes in directly fixed or cultivated brain tissue.

<p>Neurons (red), astrocytes (green) and cell nuclei (blue) were visualized with anti-NeuN antibody, anti-GFAP antibody and Hoechst, respectively, in the somatosensory cortex (layer VI) of directly fixed (30 µm) and cultivated brain tissue of neonatal (wt/tg mixed, 300 µm) and adult wild type and transgenic P301S mice (200 µm). A–C: directly fixed tissue; D–F: tissue fixed on 1^st DIV; G–I: tissue fixed on 7^th DIV; J–L: tissue fixed on 15^th DIV. A, D, G, J: neonatal tissue; B, E, H, K: transgenic (tg) adult tissue; C, F, I, L: wild type (wt) adult tissue. Scale bar 20 µm.