Immunohistochemical labeling of astrocytes (GFAP/S100B) in adult organotypic brain slices.

<p>GFAP (red), S100B (green) and cell nuclei (Hoechst, blue) in the somatosensory cortex (layer VI) of cultivated brain slices (200 µm) of adult wild type P301S mice. A–C: tissue fixed on 1^st DIV; D–F: tissue fixed on 7^th DIV; A, D: GFAP; B, E: S100B; C, F: merged images of GFAP, S100B and Hoechst. Scale bar 20 µm.