Inhibition of miR-370 activates the FOXO1 pathway. A,

<p>Relative luciferase activity assay of PC3 and DU145 cells co-transfected with the pGL3-FOXO1-3′UTR plasmid and increasing amounts (20, 50 nM) of miR-370 mimic- or miR-370 inhibitor-oligonucleotides, 48 hours after transfection, respectively. <b>B,</b> Western blotting analysis of FOXO1 expression in miR-370 or negative control (NC)-transfected PC3 and DU145 cells 48 hours after transfection. <b>C,</b> Luciferase activity assay of PC3 and DU145 cells transfected with the pGL3-FOXO1-3′UTR plasmid and increasing amounts (20, 50 nM) of miR-370 inhibitor-oligonucleotides 48 hours after transfection. Bars represent the mean ± SD values of three independent experiments; *<i>P</i><0.05.