<i>FOXO1</i> is a direct target of miR-582-5p.

<p>(A) Alignment between the predicted miR-582-5p target site of <i>FOXO1</i> 3′UTR region and miR-582-5p. (B) Real time RT-PCR and western blot analysis showing <i>FOXO1</i> mRNA and protein expression levels in THP-1 cells transfected with miR-582-5p mimics or mimics NC, respectively. (C) Co-transfection of miR-582-5p mimics/mimics NC and <i>FOXO1</i> 3′UTR-luciferase reporter vector into HEK-293T cells demonstrated that significant decrease in luciferase activity was only found in reporter vector that contained a wild type sequence (FOXO1-3′UTR-wt), not in vector that contained a mutant sequence (FOXO1-3′UTR-mut) within the miR-582-5p binding site. Values were presented as relative luciferase activity after normalization to Renilla luciferase activity. FOXO1-3′UTR-wt: pMIR-FOXO1-3′UTR-wt vector; FOXO1-3′UTR-mut: pMIR-FOXO1-3′UTR-mut vector. (D) Representative flow cytometric plots showing apoptotic ratio of THP-1 transfected with <i>FOXO1</i> siRNA or negative controls of siRNA (siRNA NC) (left panel). The apoptotic percentage of THP-1 cells transfected with <i>FOXO1</i> siRNA were significantly lower than those transfected with negative control siRNA (siRNA NC) (<i>p</i><0.01).