Supervised hierarchical clustering for ERα.

<p>Genes were filtered using analysis of variance, p-value ≤0.0001 and absolute correlation for ERα. The heat-map diagram shows the result of the two-way hierarchical clustering of miRNAs and samples. Good prognosis indicates patients with DMFS, while worse prognosis refers to patients with distant metastasis or who have died of distant metastasis. Each row represents a miRNA and each column represents a patient sample. The miRNA clustering tree is shown on the left, and the sample clustering tree appears at the top. The colour scale shown at the bottom illustrates the relative expression level of a miRNA across all samples: red colour represents an expression level above mean, blue colour represents expression lower than the mean. Gray colour means that the specific miRNA has not been successfully detected with qPCR. Numbers for clinicopathological features indicate the following: EOFUS (0 = no distant metastasis, 1 = distant metastasis), MAI10 (0<10, 1≥10), ERα (0<1% positive tumour cells, 1≥1% positive tumour cells), PPH3_13 (0<13, 1≥13), KP_Ki67 (0<10%, 1≥10%), Her2 (0 = 0 or 1+, 1 = 2+ or 3+), TNP (0 = positive for either ERα/PR/Her2, 1 = negative for ERα and PR and Her2), PR (0≤10% positive tumour cells, 1>10% positive tumour cells), CK5/6 (0 = no staining, 1 = any percentage of positive tumour cells), Tsize (Tumour size: 0≤2cm, 1>2 cm) and Nottgrade (Nottingham grade: 1 = grade 1, 2 = grade 2, 3 = grade 3).