Linkage hierarchical unsupervised clustering of differentially expressed miRNAs.

<p>a) Relative expression levels of 50 miRNAs that were expressed in all 30 matched tissue samples with Gleason score 7. The X-axis shows individuals and the Y-axis shows miRNAs. Red squares encode for up-regulated miRNAs and green squares encode for down-regulated miRNAs. b) Heat Map results of the two-way hierarchical clustering based on the qPCR results. The miRNAs showed the same trend in qPCR validation and in the microarray analysis. The normalized (dCp) values have been used for the analysis. Red color represents an expression level above mean, blue color represents expression lower than the mean.