Transgenic human αSYN immunostaining in the hippocampus of (Thy1)-h[A30P]αSYN mice.

<p>Sections from old wt, young and old (Thy1)-h[A30P]αSYN transgenic mice were stained for human transgenic αSYN with the rat monoclonal antibody 15G7. As expected staining of tissue from old wt mice did not show any signal for human αSYN throughout the whole hippocampus (A–C). Tissue from young and old transgenic mice displayed a diffuse staining in the neuropil (D–I), and old transgenic mice showed some accumulation of human transgenic αSYN in the cytoplasm of neurons (G–I). Interestingly only the synaptic regions of the CA3 (I) and especially the CA1 (H) area of old transgenic mice were positive for transgenic αSYN positive dot-like structures (arrowhead in H). This staining pattern was not observed in similar areas of young transgenic mice (E,F). Size bars correspond to 20 µm.