The sub-cellular localization of FOG-2 is not affected by SUMOylation.

<p>COS-7 cells were transfected with (A) GFP-FOG-2 or (B) GFP-FOG-2-4KR fusion proteins. The cell nuclei were stained with PI (red). There was no detectable difference in the sub-cellular or sub-nuclear distribution of wt and mutant FOG-2. COS-7 cells were co-transfected with FLAG-SENP-1 and (C) GFP-FOG-2 or (D) GFP-FOG-2-4KR fusion proteins. FLAG-SENP-1 was detected with anti-FLAG antibody and with anti mouse IgG-Alexa-594 (red). Cell nuclei were stained with DAPI (blue). The presence of FLAG-SENP-1 did not affect the sub-cellular distribution of FOG-2.