SUMOylation does not alter the subcellular localization of Oct4 and Sox2.
- Yongyan Wu (156264)
- Zekun Guo (156269)
- Haibo Wu (156272)
- Xiaohai Wang (156274)
- Lixia Yang (156282)
- Xiaoyan Shi (156294)
- Juan Du (118107)
- Bo Tang (23472)
- Wenzhong Li (156311)
- Liping Yang (156318)
- Yong Zhang (5893)
Publication date
June 2012
DOIAbstract
<p>(A) Subcellular localization of Oct4 and Oct4K118R. F9 EC cells were cotransfected with red fluorescent protein tagged Oct4/Oct4 K118R plasmids and HA-Sumo1 or HA-Ubc9. There is no obvious difference in the subcellular localization of Sumo1-modified and unmodified Oct4. (B) The distribution of Sox2 and Sox2 K247R in F9 EC cells. Cotransfection of F9 EC cells with pDsRed-<i>Sox2</i>/pDsRed-<i>Sox2</i> K247R and HA-Sumo1 or HA-Ubc9. Both SUMOylated Sox2 and unmodified Sox2 K247R localize in the nuclei. Nuclei were stained with DAPI (blue). Cells were observed and photographed under a Nikon confocal microscope at ×400 magnification.</p
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