Increased expression of LaISCL in the infective stages of <i>L. amazonensis</i>.

<p>(<b>A</b>) Promastigote lysates from <i>La</i> WT (Log: log phase; S1–S4: day 1–4 in stationary phase; Meta: metacyclics), <i>Laiscl⁻</i> (log phase), and <i>Laiscl⁻/+LaISCL</i> (log phase) were analyzed by western blot using the anti-LmISCL antibody (top) or anti-α-tubulin antibody (bottom). (<b>B</b>) Immunoblot of cell lysates from log phase <i>La</i> WT promastigotes (Pro) and lesion-derived amastigotes (Ama). Relative intensities of ISCL and tubulin bands were determined using a FluoroChem E imager and shown below the blots. Each lane contained material from 5×10⁵ cells.