Localization of LaISCL protein in promastigotes.

<p>Day 1 stationary phase promastigotes of <i>La</i> WT (left column), <i>Laiscl</i>⁻ (middle column), and <i>Laiscl</i>⁻/+<i>LaISCL</i> (right column) were analyzed by immunofluorescence microscopy. (<b>A</b>): differential interference contrast images; (<b>B</b>): DNA staining using Hoechst 33242; (<b>C</b>): immuno-staining with rabbit anti-LmISCL antibody, followed by goat-anti-rabbit IgG-FITC; (<b>D</b>): labeling with Mitotracker Red 580; (<b>E</b>): merge of <b>C</b> and <b>D</b>.