ARG absence or improper sub-cellular location impairs <i>L. amazonensis in vitro</i> infectivity.

<p>Peritoneal macrophages infected with <i>L. amazonensis</i> wild type (WT, white bars), <i>ARG</i> knockout (<i>arg</i>⁻, black bars) and the add-backs <i>arg</i>⁻/+<i>ARG</i> (grey bars) and <i>arg</i>⁻/+<i>arg</i>ΔSKL (dashed bars) mutants for 48 and 72 hours. (A) Means (+/− SD) of the rate of macrophage infection from two experiments. (B) Means (+/− SD) of amastigotes per infected macrophage from two experiments. At least 150 macrophages were analyzed for each experiment. ^# indicates values with no significant difference (One-way ANOVA). * p<0.005 and ** p<0.05 (t test).