Atg9 is inhibitory for CPP-induced autophagy.

<p>Atg9 wild type and Atg9-deficient MEFs stably expressing GFP-LC3 were treated with EBSS or CPP followed by Western blotting analysis (<b>A</b>) and quantification of GFP-LC3 puncta (<b>B–D</b>). Scale bars: 10 µm in B. Western blots shown are from one representative experiment of two to three performed for each panel. Quantitative data (mean±SEM) are from 3–5 experiments. *: <i>P</i><0.05, **: <i>P</i><0.01.