Inhibition of G9a induces autophagy in neuroblastoma cells.

<p>A, immunofluorescence analysis of five neuroblastoma cells treated with BIX01294 or water for 2 and 4 days. Scale bars, 5 µm. B, Statistical analysis of LC3B puncta in neuroblastoma cells treated with BIX01294 or water for 2 and 4 days. Each column represents the average obtained from three independent experiments; error bars, SD. Statistical analysis was performed using two-tailed student's t-test, *p≤0.01. C, western blot analysis of G9a function in neuroblastoma cells treated with BIX01294 or water for 2 days. D, western blot analysis of autophagy-related genes in neuroblastoma cells treated with BIX01294 or water for 2 days. α-Tubulin levels are shown as the loading control.