Basal autophagy levels in PC-9/wt and PC-9/gef cells.

<p>(A) Representative Western blot data showed the basal LC3-II levels in PC-9/wt and PC-9/gef cells (B4 and E3). (B) Immunofluorescent staining studies were performed using LC3 antibody to show LC3 puncta in the PC-9/wt and PC-9/gefB4 cells. (C and D): PC-9/wt, PC-9/gefB4 and PC-9/ gefE3 cells were treated with 3-methyladenine (3-MA, 10 mM) and chloroquine (CQ, 1 and 10 μM) for 24 h. Total protein of treated cells was harvested; LC3-I and II levels were analyzed with Western blot assay. Each lane contained 30 μg protein for all experiments. Results were repeated in independent experiments.