Effects of Nar and SB431542 on the expression of TGF-β/Smads classic pathways related genes.

<p>Cells were treated with 50 µM and 100 µM Nar or 10 µM and 20 µM SB431542 for 24 h before with or without 5 ng/ml TGF-β1 addition for 24 h incubation for RT-PCR or 36 h incubation for western blot. (A and B, E) The mRNA level of TβRI, TβRII, Smad2, Smad3 and Smad7 were determined by RT-PCR (A for aspc-1 cells treated by Nar, B for panc-1 cells treated by Nar, E for panc-1 cells treated by SB431542, The result was similar for aspc-1 cells, data not shown). (C and D) Value of densitometric scan was used to quantify the changes of mRNA of Smad3 and Smad7 (C for aspc-1 cells and D for panc-1 cells treated by Nar). (F and G)The level of p-Smad3 was measured by western blot. (F. a for aspc-1 cells and F. b for panc-1 cells treated with Nar, G for panc-1 cells treated with SB431542, The result was similar for aspc-1 cells, data not shown). Data were means ± SD for three individual experiments.