NPLC0393 decreased TGFβ-induced α1(I) collagen expression in HSCs.

<p>(A) LX-2 cells and the isolated primary rat HSCs were treated with increasing concentrations of NPLC0393 for 48 h. Cells were harvested for real-time PCR experiment. ***P<0.001 compared with vehicle group. (B) LX-2 cells were treated with NPLC0393 and TGFβ for 48 h, cells were then harvested and the total RNA was extracted. ^#P<0.001 compared with non-TGFβ-treated group; **P<0.01; ***P<0.001 compared with TGFβ-treated group with vehicle treatment. (C) Characterization of stable LX-2 cell line expressing shPP2Cα by western blot analysis (upper panel). Control and shPP2Cα cells were treated with increasing concentrations of NPLC0393 for 24 h and harvested for Western blotting (lower panel). (D) Control and shPP2Cα cells were treated with NPLC0393 and TGFβ for 48 h and harvested for western and real-time PCR analysis. Significant difference of the reduction on α1(I) procollagen mRNA by NPLC0393 in shPP2Cα cells versus that in control cells, *P<0.05.